In five seasons in Chicago, the 38-year-old Rozsival has played on two championship teams. He has one goal and one assist this year.
The 34-year-old Tootoo has played in 36 games after signing with Chicago last summer.
The Blackhawks announced the moves on Tuesday.
Chicago, second in the Western Conference, goes for its fifth straight win and 10th in 11 games when it hosts Pittsburgh on Wednesday.