Even against 10 men from the third division, Hamburg SV couldn't get past the German Cup's first round.
VfL Osnabruck beat the Bundesliga side 3-1 on Sunday to repeat its 2009 feat, when it defeated Hamburg on penalties.
There was no need for penalties this time as Osnabruck -- which is yet to win after four games in the third division -- again spoiled Hamburg's start to the season.
The home team played with 10 men for more than 70 minutes after defender Marcel Appiah was sent off for a foul on Bobby Wood.
Hamburg failed to make the extra man count, and Osnabruck took its first chance minutes before the break, when Nazim Sangare eluded Kyriakos Papadopoulos and Mergim Mavraj to set up Halil Savran's goal.
Hamburg coach Markus Gisdol brought on the attack-minded Lewis Holtby at the interval, but the goals fell at the other end, with Marc Heider and Ahmet Arslan extending the lead.
Wood pulled one back with a penalty for Hamburg, then missed another good chance.
Elsewhere, third-division Magdeburg upset Bundesliga team Augsburg 2-0.
Wolfsburg edged past fourth-tier Eintracht Norderstedt 1-0, thanks to Ignacio Camacho's second-half header, Leipzig beat sixth-tier Sportfreunde Dorfmerkingen 5-0, and Hannover came from behind to defeat fourth-tier side Bonner SC 6-2.
Stuttgart, back in the Bundesliga this season, progressed with a 4-3 penalty shootout win over fourth-tier Energie Cottbus.
There were also wins for second-division sides Ingolstadt, Greuther Furth and Bochum.
Union Berlin needed extra time to get past fourth-tier Saarbruecken 2-1.
SV Sandhausen was beaten 2-1 by Schweinfurt, and there was another upset when SV Wehen Wiesbaden defeated Erzgebirge Aue 2-0.