Gareth Bale has reinjured his left calf muscle and will be out indefinitely, Real Madrid said on Monday.
The soleus injury means the Wales star is likely to miss the Champions League clash on Wednesday against Paris Saint-Germain.
"Following the tests carried out on Gareth Bale in the Sanitas la Moraleja University Hospital, the player has been diagnosed with a soleus muscle injury in his left leg. His recovery will continue to be assessed," the club said on their website.
Bale limped out of last month's 4-0 Champions League Group A win at home to Shakhtar Donetsk with a calf injury, the fourth time in just two years he has injured the leg.
Bale played 45 minutes in Real Madrid's 3-0 win over Levante and was substituted by Lucas Vazquez. On Sunday he went to a private clinic for treatment, AS reported, and on Monday he did not train with the team.
Bale played in both of Wales' historic Euro 2016 qualifying matches last week against Bosnia-Herzegovina and Andorra. Qualification came despite a 2-0 defeat in Bosnia, the first reverse in 11 competitive matches, but Wales still made it to France next summer after Cyprus had ended Israel's hopes of a Group B top-two spot with a 2-1 win in Jerusalem.
Real Madrid coach Rafa Benitez admitted after Saturday's match that Bale was tired.
"After the 2-0 score and with the intense week he had, we saw that he was tired and that was the moment to put fresh legs in the match," Benitez said.
AS reported that Bale is likely to miss both the Champions League match as well as Saturday's La Liga game against Celta Vigo.