A last-gasp equaliser from Leonardo Blanchard denied Juventus victory and earned Frosinone a historic first point in Serie A on Wednesday.
Juve, who have yet to win at home, looked to be heading for their second victory of their campaign in the 50th minute when Simone Zaza struck his first goal since joining the Turin giants from Sassuolo this summer.
However, Blanchard silenced the crowd at the Juventus stadium when he rose highest to head Danilo Soddimo's corner into the back of the net with one minute remaining.
Juve were without five injured players, including striker Alvaro Morata, from the side that won 2-0 at Genoa on Sunday, the Bianconeri's only league victory so far.
Zaza earned his first start while Juve rested veteran Gianluigi Buffon, with Neto playing in goal.
Frosinone, in their maiden Serie A campaign, almost went in front in the 30th minute when Nicolas Castillo struck a right-footed shot that hit the left post with Neto rooted to the spot.
Frosinone demanded a penalty soon after when Andrea Barzagli looked to have used his arm to deflect Alessandro Frara's effort but the referee waved play on.
The hosts were unlucky not to move in front in the 40th minute when Paul Pogba's header from Roberto Pereyra's cross rattled the crossbar.
Four minutes after the restart, a Frosinone defender deflected Zaza's header off the woodwork.
Juve broke the deadlock seconds later when Zaza struck a right-footed shot into the net only for Frosinone to get a late equaliser.