The Australian hit some underhand serves, including one that landed in for an ace. He tried a few 'tweeners. And he even ran in early on a serve from Nadal, and argued loudly with the chair umpire.
The antics were entertaining, but not enough to eliminate the two-time champion from Spain, who won 6-3, 3-6, 7-6 (5), 7-6 (3) on Centre Court to reach the third round.
Nadal and Kyrgios don't have the best relationship on the court or off it. In 2014, a 19-year-old Kyrgios beat Nadal at Wimbledon to become the youngest man to eliminate the No. 1 player at a major tournament since 2005. Add to that a public spat involving Nadal, his uncle and Kyrgios in recent months.
"Potentially," Nadal said of the 43rd-ranked Kyrgios, "he's a Grand Slam winner.
"When he wants to compete well, he's one of the toughest opponents you can face. Normally against me and the top guys, he wants to try hard. And when he's that way, he's very tough."
After his second-round victory, Kyrgios said he wouldn't expect to "have a beer together'' at the Dog & Fox, a nearby pub where the 24-year-old Australian was seen Wednesday night.
The 33-year-old Nadal responded in a separate interview by saying he was "too old for all this stuff.''
The No. 2-seeded Nadal is seeking his third Wimbledon title but first since 2010. He is eyeing a 19th Grand Slam title overall, which would move him within one of Roger Federer's record for men.
While Nadal had to work hard to reach the third round, Federer, an eight-time champion at the All-England Club, dominated British wild-card entry Jay Clarke 6-1, 7-6 (3), 6-2 in a rare visit to No. 1 Court.
Clarke is ranked No. 169, but he held his own against Federer in the second set until the tiebreaker, when the Swiss star won four straight points to take a 5-2 lead. He then jumped out a 3-0 lead in the third set and broke again in the final game.
Last year, Federer lost his only match on No. 1 Court to Kevin Anderson in the quarterfinals after wasting a two-set lead. But he said the new roof over the stadium made it feel more like his usual surroundings.
"I really enjoyed myself on Court 1 today with the roof," Federer said. "I couldn't really tell if it was Centre Court or Court 1, actually."
The hard-serving American was one set away from the victory but lost 6-4, 6-7 (3), 4-6, 6-1, 6-4 on No. 3 Court. Kukushkin broke in the opening game of the fifth set and held serve the rest of the way, converting his first match point when the ninth-seeded Isner sent a backhand long.
Isner reached his first Grand Slam semifinal at Wimbledon last year before losing a five-setter to Anderson that lasted more than 6½ hours, the second-longest match in Grand Slam history. Isner also was involved in the longest, beating Nicolas Mahut in an 11-hour match that finished 70-68 in the fifth set at Wimbledon in 2010.
Johnson, who is ranked 71st, had lost his past nine matches anywhere against men inside the ATP's top 30.
How close was this one? Johnson won fewer total points (150-149), earned fewer service breaks (3-2) and made nearly twice as many unforced errors (45-25).
But Johnson broke de Minaur, who is ranked 29th, in the next-to-last game then served out the victory, which ended when Johnson's down-the-line forehand drew a long backhand on a 22-stroke exchange.
Johnson's best Grand Slam showing was a fourth-round run at the All England Club in 2016, which ended with a loss to Federer.
The Associated Press contributed to this report.