Nico O'Reilly will return to Manchester City and play no further part in England's upcoming Nations League games after he picked up an injury.
The left back played all of England's defeat to Spain in their opening game of the international break, but was absent from Tuesday's 2-0 win against Czechia having missed training in the buildup to the game.
In a statement, England said on Thursday that the City defender was returning to his club for "further assessment."
The statement added that no further replacements were planned to join up with the squad.